Thermal and gastric stability of antimicrobial activity of juices and aqueous extracts prepared from common eligible herbs and traditional medicinal plants against Burkholderia pseudomallei and other enteric bacteria

Abstract Background Burkholderia pseudomallei is a causative agent of melioidosis, fatal infectious disease highly prevalent in the tropics where traditional medicinal plants are widely used for treatment various human ailments. In this study, we aimed to evaluate vitro antibacterial activity common eligible herbs and against B. . Thermal gastric stability, spectrum, bactericidal activity, cell cytotoxicity were also tested verify possible usage these melioidosis. Results Eighteen twenty-one collected. Herb juices aqueous plant samples extracted at different temperatures prepared testing. A higher proportion extracts (17/21; 80.9%) was observed, comparison with that herb (8/18; 44.5%). Two twelve selected further tests. The A. sativum tuberosum decreased their antimicrobial when treated whereas increased 70 100 °C. showed broader spectrum than extracts. All less on HT29, HepG2, HEK293 lines. At 2× minimal inhibitory concentration (MIC), Blechnum orientale , Breynia fruticose, Psidium guajava Rhodomyrtus tomentosa Rosa odorata Schima wallichii similar amoxicillin clavulanic acid. Mangifera indica Punica granatum R. remained under stimulated conditions. Conclusion Our data indicate by decoction could be effectively treat melioidosis via oral route. Further vivo investigations needed explore other alternative therapies prevention pathogenic bacterial species.